Assume your tilapia fish muscle homogenate has a protein concentration of 5769 ?g/ml. You take 32.5 ?l of this fish homogenate, mix it with 12.5 ?l sample buffer and 5 ?l reducing agent, and incubate 10 minutes at 70°C. How much of this “gel-ready” solution must you pipette into a well of your electrophoresis gel to load 60 ?g of protein?
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